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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2214 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2214, RRID:AB_1577073
- Product name
- MBD3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MBD3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase.
A mammalian protein with specific demethylase activity for mCpG DNA.
Identification and characterization of a family of mammalian methyl-CpG binding proteins.
Petronzelli F, Riccio A, Markham GD, Seeholzer SH, Stoerker J, Genuardi M, Yeung AT, Matsumoto Y, Bellacosa A
The Journal of biological chemistry 2000 Oct 20;275(42):32422-9
The Journal of biological chemistry 2000 Oct 20;275(42):32422-9
A mammalian protein with specific demethylase activity for mCpG DNA.
Bhattacharya SK, Ramchandani S, Cervoni N, Szyf M
Nature 1999 Feb 18;397(6720):579-83
Nature 1999 Feb 18;397(6720):579-83
Identification and characterization of a family of mammalian methyl-CpG binding proteins.
Hendrich B, Bird A
Molecular and cellular biology 1998 Nov;18(11):6538-47
Molecular and cellular biology 1998 Nov;18(11):6538-47
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MBD3 (arrow) using rabbit MBD3 polyclonal antibody (Cat # PAB2214). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MBD3 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MBD3 polyclonal antibody (Cat # PAB2214) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
