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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA1-41126 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MBD3 Monoclonal Antibody (106B691)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with rat and mouse based on sequence homology. Suggested positive control: HeLa nuclear extract.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 106B691
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Refinement of the subunit interaction network within the nucleosome remodelling and deacetylase (NuRD) complex.
Torrado M, Low JKK, Silva APG, Schmidberger JW, Sana M, Sharifi Tabar M, Isilak ME, Winning CS, Kwong C, Bedward MJ, Sperlazza MJ, Williams DC Jr, Shepherd NE, Mackay JP
The FEBS journal 2017 Dec;284(24):4216-4232
The FEBS journal 2017 Dec;284(24):4216-4232
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MBD3 in HeLa cell lysate (nuclear fraction) in the A) absence and B) presence of immunizing peptide. Samples were incubated in MBD3 monoclonal antibody (Product # MA1-41126) using a dilution of 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MBD3 in immersion fixed paraffin-embedded sections of human placenta. Samples were incubated in MBD3 monoclonal antibody (Product # MA1-41126) using a dilution of 1:300 for 1 hour at room temperature followed by the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in horionic villi.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
