Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-17193 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TUBA8 Monoclonal Antibody (2D6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17193 targets TUBA8 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Rat samples.
- Antibody clone number
- 2D6
- Concentration
- Conc. Not Determined
Submitted references Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies.
Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis.
Cortese M, Lee JY, Cerikan B, Neufeldt CJ, Oorschot VMJ, Köhrer S, Hennies J, Schieber NL, Ronchi P, Mizzon G, Romero-Brey I, Santarella-Mellwig R, Schorb M, Boermel M, Mocaer K, Beckwith MS, Templin RM, Gross V, Pape C, Tischer C, Frankish J, Horvat NK, Laketa V, Stanifer M, Boulant S, Ruggieri A, Chatel-Chaix L, Schwab Y, Bartenschlager R
Cell host & microbe 2020 Dec 9;28(6):853-866.e5
Cell host & microbe 2020 Dec 9;28(6):853-866.e5
Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis.
Hamel V, Steib E, Hamelin R, Armand F, Borgers S, Flückiger I, Busso C, Olieric N, Sorzano COS, Steinmetz MO, Guichard P, Gönczy P
Current biology : CB 2017 Aug 21;27(16):2486-2498.e6
Current biology : CB 2017 Aug 21;27(16):2486-2498.e6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TUBA8 using a TUBA8 monoclonal antibody (Product # MA5-17193) against a human TUBA8 (AA: 294-449) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), A549 (Lane 2), COS-7 (Lane 3), MDCK (Lane 4), NIH/3T3 (Lane 5), PC-12 (Lane 6), Neuro-2a (Lane 7), tissue extracts of Mouse Brain (Lane 9) and Rat Testis (Lane 10). The blot was probed with Anti-TUBA8 Monoclonal Antibody (2D6) (Product # MA5-17193, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 52 kDa band corresponding to alpha Tubulin was observed across the cell lines and tissues tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TUBA8 using TUBA8 monoclonal antibody (Product # MA5-17193) in rat heart (1) tissue lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of TUBA8 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TUBA8 Monoclonal Antibody (2D6) (Product # MA5-17193) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using TUBA8 monoclonal antibody (Product # MA5-17193) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using TUBA8 monoclonal antibody (Product # MA5-17193) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TUBA8 monoclonal antibody (Product # MA5-17193) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using TUBA8 monoclonal antibody (Product # MA5-17193) (green) and negative control (red).