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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501038 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501038, RRID:AB_11128580
- Product name
- Anti-TUBA8 mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)
- Antibody type
- Monoclonal
- Description
- Anti-TUBA8 mouse monoclonal antibody, clone OTI2E9 (formerly 2E9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TUBA8
- Isotype
- IgG
- Antibody clone number
- OTI2E9
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA8 (RC211175, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA8.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-TUBA8 mouse monoclonal antibody (TA501038) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBA8(RC211175).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of TUBA8 by using TrueMab monoclonal anti-TUBA8 antibodies (Negative control: IP without adding anti-TUBA8 antibody.). For each experiment, 500ul of DDK tagged TUBA8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY TUBA8(RC211175)(Red) or empty vector control plasmid(Blue) were immunostained with anti-TUBA8 mouse monoclonal(TA501038), and then analyzed by flow cytometry.
- Validation comment
- FC
