- PA5-29135 - Invitrogen Antibodies TUBA8 antibody

Submitted references Human IDO-competent, long-lived immunoregulatory dendritic cells induced by intracellular pathogen, and their fate in humanized mice.

Tyagi RK, Miles B, Parmar R, Garg NK, Dalai SK, Baban B, Cutler CW
Scientific reports 2017 Feb 15;7:41083

Nanostructured lipid carrier mediates effective delivery of methotrexate to induce apoptosis of rheumatoid arthritis via NF-κB and FOXO1.

Garg NK, Tyagi RK, Singh B, Sharma G, Nirbhavane P, Kushwah V, Jain S, Katare OP
International journal of pharmaceutics 2016 Feb 29;499(1-2):301-320

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of TUBA8 was performed by separating 30 µg of various whole cell extracts by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a TUBA8 Polyclonal Antibody (Product # PA5-29135) at a dilution of 1:1000. A: A431, B: Hela.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), MDA-MB-231 (Lane 5), PC-12 (Lane 6), RSC96 (Lane 7) and tissue extracts of Mouse Lung (Lane 8). The blot was probed with Anti-TUBA8 Rabbit Polyclonal Antibody (Product # PA5-29135, 1:2500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 52 kDa band corresponding to TUBA8 was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of TUBA8 was performed by separating 30 µg of NIH-3T3 lysates by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a TUBA8 Polyclonal Antibody (Product # PA5-29135) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of TUBA8 was performed by separating 30 µg of various whole cell extracts by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a TUBA8 Polyclonal Antibody (Product # PA5-29135) at a dilution of 1:1000. A: A431, B: Hela.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of alpha Tubulin 8 in paraformaldehyde-fixed A431 cells using an Alpha Tubulin 8 polyclonal antibody (Product # PA5-29135) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of TUBA8 was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TUBA8 Rabbit Polyclonal Antibody (Product # PA5-29135) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of TUBA8 in paraformaldehyde-fixed A431 cells using TUBA8 Polyclonal Antibody (Product # PA5-29135) at a dilution of 1:200.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin 8 (Product # PA5-29135) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Phosphorylated Foxo1 decreases with PDDC apoptosis rate. ( A ) FOXO1 expression was observed on protein level in PDDCs (WT & DPG) by carrying out Western blot using Anti-FOXO1 Mab. alpha-Tubulin, loading control (n = 2). The amount of total PDDC Foxo1 ( B ) and phosphorylated Foxo1 ( C ) were measured by whole cell ELISA and normalized to total cell numbers (n = 3) (B) PDDCs generated with E. coli LPS or either of the P. gingivalis strains have significantly higher levels of Foxo1. ( C ) PDDCs generated by the minor-fimbriae expressing WT and DPG-3 or the non-fimbriated MFB have significantly higher levels of phosphorylated Foxo1 ( D ) DCs were washed in RPMI and then incubated for 10 hrs in 10% FBS in RPMI. DCs were then lysed and Bim was detected by immunoblot. alpha-tubulin, loading control (n = 2).

PA5-29135

Antibody data