Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB10241 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10241, RRID:AB_1716843
- Product name
- USP18 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of USP18.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The ISG15 isopeptidase UBP43 is regulated by proteolysis via the SCFSkp2 ubiquitin ligase.
Human and mouse proteases: a comparative genomic approach.
Cloning and characterization of human ubiquitin-processing protease-43 from terminally differentiated human melanoma cells using a rapid subtraction hybridization protocol RaSH.
RNase-L-dependent destabilization of interferon-induced mRNAs. A role for the 2-5A system in attenuation of the interferon response.
Cloning and characterization of a novel human ubiquitin-specific protease, a homologue of murine UBP43 (Usp18).
Tokarz S, Berset C, La Rue J, Friedman K, Nakayama K, Nakayama K, Zhang DE, Lanker S
The Journal of biological chemistry 2004 Nov 5;279(45):46424-30
The Journal of biological chemistry 2004 Nov 5;279(45):46424-30
Human and mouse proteases: a comparative genomic approach.
Puente XS, Sánchez LM, Overall CM, López-Otín C
Nature reviews. Genetics 2003 Jul;4(7):544-58
Nature reviews. Genetics 2003 Jul;4(7):544-58
Cloning and characterization of human ubiquitin-processing protease-43 from terminally differentiated human melanoma cells using a rapid subtraction hybridization protocol RaSH.
Kang D, Jiang H, Wu Q, Pestka S, Fisher PB
Gene 2001 Apr 18;267(2):233-42
Gene 2001 Apr 18;267(2):233-42
RNase-L-dependent destabilization of interferon-induced mRNAs. A role for the 2-5A system in attenuation of the interferon response.
Li XL, Blackford JA, Judge CS, Liu M, Xiao W, Kalvakolanu DV, Hassel BA
The Journal of biological chemistry 2000 Mar 24;275(12):8880-8
The Journal of biological chemistry 2000 Mar 24;275(12):8880-8
Cloning and characterization of a novel human ubiquitin-specific protease, a homologue of murine UBP43 (Usp18).
Schwer H, Liu LQ, Zhou L, Little MT, Pan Z, Hetherington CJ, Zhang DE
Genomics 2000 Apr 1;65(1):44-52
Genomics 2000 Apr 1;65(1):44-52
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using USP18 polyclonal antibody (Cat # PAB10241) shows detection of a band ~43 KDa band (arrowhead) believed to be to USP18 in lysates from HeLa nuclear extracts (Lane 1) and whole cell lysates from HeLa (Lane 2), A-431 (Lane 3), Jurkat (Lane 4) and HEK293 (Lane 5).Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of USP18 polyclonal antibody.Signal was detected using standard techniques.The strong band between the 37 KDa and 50 KDa markers is expected to be USP18.The other bands are probably non-specific.
