MA5-36231

antibody from Invitrogen Antibodies

Targeting: HSPH1 HSP105A, HSP105B, KIAA0201, NY-CO-25

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-36231

Provider

Invitrogen Antibodies

Product name

HSPH1 Monoclonal Antibody (3D10)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3D10

Vial size

100 µg

Concentration

500 µg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HSPH1 in the following samples: Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Tween (3 times, 5 minutes/wash) and blocked with 5% Non-fat Milk/TBS (1.5 hrs., room temperature). The membrane was incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 0.5 µg/mL (overnight, 4°C), followed by goat anti-mouse IgG-HRP and chemiluminescence (ECL) with a dilution of 1:10,000 (1.5 hours, room temperature).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HSPH1 in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HSPH1 in A431 cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HSPH1 in paraffin-embedded human mammary cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HSPH1 in paraffin-embedded human lung cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HSPH1 in paraffin-embedded human intestinal cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HSPH1 in HepG2 cells. Samples were blocked with 10% normal goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) and unlabeled sample (Red line).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HSPH1 in A431 cells. Samples were blocked with 10% normal goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x10^6) and unlabeled sample (Red line).