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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4029 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat HSPH1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat HSPH1. This antibody is predicted to react with HSPH1 alpha and beta isoforms based on sequence identity of the immunogen.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q66HA8- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat HSPH1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, DA3 mouse myeloma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse/Rat HSPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4029) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSPH1 at approximately 105-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HSPH1 in Rat Brain. HSPH1 was detected in perfusion fixed frozen sections of rat brain (cerebellum) using 5 µg/mL Human/Mouse/Rat HSPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4029) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
