Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1828 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TREM2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TREM2 in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross-reactivity with recombinant mouse TREM2b is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model.
Glial activation and inflammation along the Alzheimer's disease continuum.
CSF sTREM2 in delirium-relation to Alzheimer's disease CSF biomarkers Aβ42, t-tau and p-tau.
Small nuclear RNA-mediated modulation of splicing reveals a therapeutic strategy for a TREM2 mutation and its post-transcriptional regulation.
Elevated TREM2 Gene Dosage Reprograms Microglia Responsivity and Ameliorates Pathological Phenotypes in Alzheimer's Disease Models.
TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function.
Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis.
TREM2 shedding by cleavage at the H157-S158 bond is accelerated for the Alzheimer's disease-associated H157Y variant.
An Alzheimer-associated TREM2 variant occurs at the ADAM cleavage site and affects shedding and phagocytic function.
A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells.
Intracellular trafficking of TREM2 is regulated by presenilin 1.
TREM-2 promotes acquired cholesteatoma-induced bone destruction by modulating TLR4 signaling pathway and osteoclasts activation.
Inhibition of RANK expression and osteoclastogenesis by TLRs and IFN-gamma in human osteoclast precursors.
IL-10 suppresses calcium-mediated costimulation of receptor activator NF-kappa B signaling during human osteoclast differentiation by inhibiting TREM-2 expression.
TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells.
Zhong L, Xu Y, Zhuo R, Wang T, Wang K, Huang R, Wang D, Gao Y, Zhu Y, Sheng X, Chen K, Wang N, Zhu L, Can D, Marten Y, Shinohara M, Liu CC, Du D, Sun H, Wen L, Xu H, Bu G, Chen XF
Nature communications 2019 Mar 25;10(1):1365
Nature communications 2019 Mar 25;10(1):1365
Glial activation and inflammation along the Alzheimer's disease continuum.
Nordengen K, Kirsebom BE, Henjum K, Selnes P, Gísladóttir B, Wettergreen M, Torsetnes SB, Grøntvedt GR, Waterloo KK, Aarsland D, Nilsson LNG, Fladby T
Journal of neuroinflammation 2019 Feb 21;16(1):46
Journal of neuroinflammation 2019 Feb 21;16(1):46
CSF sTREM2 in delirium-relation to Alzheimer's disease CSF biomarkers Aβ42, t-tau and p-tau.
Henjum K, Quist-Paulsen E, Zetterberg H, Blennow K, Nilsson LNG, Watne LO
Journal of neuroinflammation 2018 Nov 3;15(1):304
Journal of neuroinflammation 2018 Nov 3;15(1):304
Small nuclear RNA-mediated modulation of splicing reveals a therapeutic strategy for a TREM2 mutation and its post-transcriptional regulation.
Yanaizu M, Sakai K, Tosaki Y, Kino Y, Satoh JI
Scientific reports 2018 May 2;8(1):6937
Scientific reports 2018 May 2;8(1):6937
Elevated TREM2 Gene Dosage Reprograms Microglia Responsivity and Ameliorates Pathological Phenotypes in Alzheimer's Disease Models.
Lee CYD, Daggett A, Gu X, Jiang LL, Langfelder P, Li X, Wang N, Zhao Y, Park CS, Cooper Y, Ferando I, Mody I, Coppola G, Xu H, Yang XW
Neuron 2018 Mar 7;97(5):1032-1048.e5
Neuron 2018 Mar 7;97(5):1032-1048.e5
TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function.
Zhao Y, Wu X, Li X, Jiang LL, Gui X, Liu Y, Sun Y, Zhu B, Piña-Crespo JC, Zhang M, Zhang N, Chen X, Bu G, An Z, Huang TY, Xu H
Neuron 2018 Mar 7;97(5):1023-1031.e7
Neuron 2018 Mar 7;97(5):1023-1031.e7
Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis.
Garcia-Reitboeck P, Phillips A, Piers TM, Villegas-Llerena C, Butler M, Mallach A, Rodrigues C, Arber CE, Heslegrave A, Zetterberg H, Neumann H, Neame S, Houlden H, Hardy J, Pocock JM
Cell reports 2018 Aug 28;24(9):2300-2311
Cell reports 2018 Aug 28;24(9):2300-2311
TREM2 shedding by cleavage at the H157-S158 bond is accelerated for the Alzheimer's disease-associated H157Y variant.
Thornton P, Sevalle J, Deery MJ, Fraser G, Zhou Y, Ståhl S, Franssen EH, Dodd RB, Qamar S, Gomez Perez-Nievas B, Nicol LS, Eketjäll S, Revell J, Jones C, Billinton A, St George-Hyslop PH, Chessell I, Crowther DC
EMBO molecular medicine 2017 Oct;9(10):1366-1378
EMBO molecular medicine 2017 Oct;9(10):1366-1378
An Alzheimer-associated TREM2 variant occurs at the ADAM cleavage site and affects shedding and phagocytic function.
Schlepckow K, Kleinberger G, Fukumori A, Feederle R, Lichtenthaler SF, Steiner H, Haass C
EMBO molecular medicine 2017 Oct;9(10):1356-1365
EMBO molecular medicine 2017 Oct;9(10):1356-1365
A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells.
Varnum MM, Clayton KA, Yoshii-Kitahara A, Yonemoto G, Koro L, Ikezu S, Ikezu T
The Journal of biological chemistry 2017 Jun 23;292(25):10651-10663
The Journal of biological chemistry 2017 Jun 23;292(25):10651-10663
Intracellular trafficking of TREM2 is regulated by presenilin 1.
Zhao Y, Li X, Huang T, Jiang LL, Tan Z, Zhang M, Cheng IH, Wang X, Bu G, Zhang YW, Wang Q, Xu H
Experimental & molecular medicine 2017 Dec 1;49(12):e405
Experimental & molecular medicine 2017 Dec 1;49(12):e405
TREM-2 promotes acquired cholesteatoma-induced bone destruction by modulating TLR4 signaling pathway and osteoclasts activation.
Jiang H, Si Y, Li Z, Huang X, Chen S, Zheng Y, Xu G, Chen X, Chen Y, Liu Y, Xiong H, Huang Q, Liang M, Zhang Z
Scientific reports 2016 Dec 9;6:38761
Scientific reports 2016 Dec 9;6:38761
Inhibition of RANK expression and osteoclastogenesis by TLRs and IFN-gamma in human osteoclast precursors.
Ji JD, Park-Min KH, Shen Z, Fajardo RJ, Goldring SR, McHugh KP, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 1;183(11):7223-33
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 1;183(11):7223-33
IL-10 suppresses calcium-mediated costimulation of receptor activator NF-kappa B signaling during human osteoclast differentiation by inhibiting TREM-2 expression.
Park-Min KH, Ji JD, Antoniv T, Reid AC, Silver RB, Humphrey MB, Nakamura M, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 15;183(4):2444-55
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 15;183(4):2444-55
TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells.
Quan DN, Cooper MD, Potter JL, Roberts MH, Cheng H, Jarvis GA
Mucosal immunology 2008 May;1(3):229-38
Mucosal immunology 2008 May;1(3):229-38
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human TREM2 by Western Blot. Detection of Human TREM2 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Human TREM2 ELISA Standard Curve. Human TREM2 ELISA Standard Curve.
