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- Product number
- AF1729 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TREM2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse TREM2 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant human TREM2 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q99NH8- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references APOE genotype and sex affect microglial interactions with plaques in Alzheimer's disease mice.
Elevated TREM2 Gene Dosage Reprograms Microglia Responsivity and Ameliorates Pathological Phenotypes in Alzheimer's Disease Models.
TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function.
The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease.
Gene co-expression networks identify Trem2 and Tyrobp as major hubs in human APOE expressing mice following traumatic brain injury.
MicroRNA-101a regulates microglial morphology and inflammation.
Effect of high fat diet on phenotype, brain transcriptome and lipidome in Alzheimer's model mice.
Intracellular trafficking of TREM2 is regulated by presenilin 1.
Deficiency of a sulfotransferase for sialic acid-modified glycans mitigates Alzheimer's pathology.
Differential modulation of TREM2 protein during postnatal brain development in mice.
In situ dividing and phagocytosing retinal microglia express nestin, vimentin, and NG2 in vivo.
Stephen TL, Cacciottolo M, Balu D, Morgan TE, LaDu MJ, Finch CE, Pike CJ
Acta neuropathologica communications 2019 May 21;7(1):82
Acta neuropathologica communications 2019 May 21;7(1):82
Elevated TREM2 Gene Dosage Reprograms Microglia Responsivity and Ameliorates Pathological Phenotypes in Alzheimer's Disease Models.
Lee CYD, Daggett A, Gu X, Jiang LL, Langfelder P, Li X, Wang N, Zhao Y, Park CS, Cooper Y, Ferando I, Mody I, Coppola G, Xu H, Yang XW
Neuron 2018 Mar 7;97(5):1032-1048.e5
Neuron 2018 Mar 7;97(5):1032-1048.e5
TREM2 Is a Receptor for β-Amyloid that Mediates Microglial Function.
Zhao Y, Wu X, Li X, Jiang LL, Gui X, Liu Y, Sun Y, Zhu B, Piña-Crespo JC, Zhang M, Zhang N, Chen X, Bu G, An Z, Huang TY, Xu H
Neuron 2018 Mar 7;97(5):1023-1031.e7
Neuron 2018 Mar 7;97(5):1023-1031.e7
The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease.
Cheng-Hathaway PJ, Reed-Geaghan EG, Jay TR, Casali BT, Bemiller SM, Puntambekar SS, von Saucken VE, Williams RY, Karlo JC, Moutinho M, Xu G, Ransohoff RM, Lamb BT, Landreth GE
Molecular neurodegeneration 2018 Jun 1;13(1):29
Molecular neurodegeneration 2018 Jun 1;13(1):29
Gene co-expression networks identify Trem2 and Tyrobp as major hubs in human APOE expressing mice following traumatic brain injury.
Castranio EL, Mounier A, Wolfe CM, Nam KN, Fitz NF, Letronne F, Schug J, Koldamova R, Lefterov I
Neurobiology of disease 2017 Sep;105:1-14
Neurobiology of disease 2017 Sep;105:1-14
MicroRNA-101a regulates microglial morphology and inflammation.
Saika R, Sakuma H, Noto D, Yamaguchi S, Yamamura T, Miyake S
Journal of neuroinflammation 2017 May 30;14(1):109
Journal of neuroinflammation 2017 May 30;14(1):109
Effect of high fat diet on phenotype, brain transcriptome and lipidome in Alzheimer's model mice.
Nam KN, Mounier A, Wolfe CM, Fitz NF, Carter AY, Castranio EL, Kamboh HI, Reeves VL, Wang J, Han X, Schug J, Lefterov I, Koldamova R
Scientific reports 2017 Jun 27;7(1):4307
Scientific reports 2017 Jun 27;7(1):4307
Intracellular trafficking of TREM2 is regulated by presenilin 1.
Zhao Y, Li X, Huang T, Jiang LL, Tan Z, Zhang M, Cheng IH, Wang X, Bu G, Zhang YW, Wang Q, Xu H
Experimental & molecular medicine 2017 Dec 1;49(12):e405
Experimental & molecular medicine 2017 Dec 1;49(12):e405
Deficiency of a sulfotransferase for sialic acid-modified glycans mitigates Alzheimer's pathology.
Zhang Z, Takeda-Uchimura Y, Foyez T, Ohtake-Niimi S, Narentuya, Akatsu H, Nishitsuji K, Michikawa M, Wyss-Coray T, Kadomatsu K, Uchimura K
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 4;114(14):E2947-E2954
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 4;114(14):E2947-E2954
Differential modulation of TREM2 protein during postnatal brain development in mice.
Chertoff M, Shrivastava K, Gonzalez B, Acarin L, Giménez-Llort L
PloS one 2013;8(8):e72083
PloS one 2013;8(8):e72083
In situ dividing and phagocytosing retinal microglia express nestin, vimentin, and NG2 in vivo.
Wohl SG, Schmeer CW, Friese T, Witte OW, Isenmann S
PloS one 2011;6(8):e22408
PloS one 2011;6(8):e22408
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Mouse TREM2 ELISA Standard Curve. Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TREM2 in RAW 264.7 Mouse Cell Line. TREM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
