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- Product number
- NBP1-07101 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-07101, RRID:AB_1625511
- Product name
- Mouse Monoclonal TREM2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Trem2 Splicing and Expression are Preserved in a Human Aβ-producing, Rat Knock-in Model of Trem2-R47H Alzheimer's Risk Variant.
The triggering receptor expressed on myeloid cells 2 (TREM2) is associated with enhanced inflammation, neuropathological lesions and increased risk for Alzheimer's dementia.
A survey of TREM2 antibodies reveals neuronal but not microglial staining in formalin-fixed paraffin-embedded postmortem Alzheimer's brain tissues.
Tambini MD, D'Adamio L
Scientific reports 2020 Mar 5;10(1):4122
Scientific reports 2020 Mar 5;10(1):4122
The triggering receptor expressed on myeloid cells 2 (TREM2) is associated with enhanced inflammation, neuropathological lesions and increased risk for Alzheimer's dementia.
Roussos P, Katsel P, Fam P, Tan W, Purohit DP, Haroutunian V
Alzheimer's & dementia : the journal of the Alzheimer's Association 2015 Oct;11(10):1163-70
Alzheimer's & dementia : the journal of the Alzheimer's Association 2015 Oct;11(10):1163-70
A survey of TREM2 antibodies reveals neuronal but not microglial staining in formalin-fixed paraffin-embedded postmortem Alzheimer's brain tissues.
Satoh J, Kawana N, Yamamoto Y, Ishida T, Saito Y, Arima K
Alzheimer's research & therapy 2013;5(4):30
Alzheimer's research & therapy 2013;5(4):30
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: TREM-2 Antibody (2B5) [NBP1-07101] - Simple Western lane view shows a specific band for TREM-2 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TREM-2 Antibody (2B5) [NBP1-07101] - Cell lysates of HeLa, MCF7, LNCaP and mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TREM2 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TREM-2 Antibody (2B5) [NBP1-07101] - Flow cytometry analysis of TREM2 in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
