Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ANR-018-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TREM2 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse BV-2 microglia (lanes 1 and 4), human U-87 MG glioblastoma (lanes 2 and 5) and human THP-1 monocytic leukemia (lanes 3 and 6)cell line lysates: - 1-3. Anti-TREM2 (extracellular) Antibody (#ANR-018), (1:200).4-6. Anti-TREM2 (extracellular) Antibody , preincubated with TREM2 (extracellular) Blocking Peptide (#BLP-NR018).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain lysates: - 1,2. Anti-TREM2 (extracellular) Antibody (#ANR-018), (1:200).3,4. Anti-TREM2 (extracellular) Antibody , preincubated with TREM2 (extracellular) Blocking Peptide (#BLP-NR018).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression ofTREM2in mouse hippocampus in akainicacidneurodegenerationmodel. Immunohistochemicalstaining of perfusion-fixed frozen mouse brain sectionscollected4 days postkainateinjection with Anti-TREM2 (extracellular) Antibody (#ANR-018), (1:300), followed by goat Anti-rabbit-AlexaFluor-488.A. Staining in the mousehippocampal CA1 regionshowedTREM2immunoreactivity (green)inastrocytes inthestratumoriens (SO) and the stratumradiatum (SR).B. Pre-incubation of the Antibody with TREM2 (extracellular)Blocking Peptide (BLP-NR018), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of TREM2 in live intact mouse J774 macrophage cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-TREM2 (extracellular) Antibody (#ANR-018), (2.5 µg) + goat- Anti-rabbit-FITC.