MAB2243-100
antibody from R&D Systems
Targeting: TMED1
Il1rl1l, IL1RL1LG, MGC1270, p24g1, p24gamma1, ST2L
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB2243-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TMED1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TMED1 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q13445
- Isotype
- IgG
- Antibody clone number
- 1009527
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TMED1 in A549 Human Cell Line. TMED1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TMED1 Monoclonal Antibody (Catalog # MAB2243) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TMED-1 in HEK293 Human Cell Line Transfected with Human TMED-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) TMED-1 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human TMED-1 Monoclonal Antibody (Catalog # MAB2243) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.