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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- GTX48823 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48823, RRID:AB_11164035
- Product name
- REXO1 antibody [4H5]
- Antibody type
- Monoclonal
- Host
- Mouse
- Vial size
- 100 µl
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Anti-Rub1 antibody, generated by immunization with full-length, recombinant yeast Rub1, was tested by western blot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG
- Validation comment
- WB
