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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-58818 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FBXL16 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ILNGLFWYFS ACEKCVLAQV CKAWRRVLYQ PKFWAGLTPV LHAKELYNVL PGGEKEFVNL QGFAARGFEG FCLVGVSDLD ICEFIDNYAL SKKGVKA
- Concentration
- 0.05 mg/mL
Submitted references FBXL16 Promotes Endometrial Progesterone Resistance via PP2A(B55α) /Cyclin D1 Axis in Ishikawa.
Liu H, Han L, Zhong L, Zhuang X, Peng Y
Journal of immunology research 2022;2022:7372202
Journal of immunology research 2022;2022:7372202
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FBXL16 in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cerebral cortex tissue. Samples were probed using a FBXL16 Polyclonal Antibody (Product # PA5-58818).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FBXL16 in control (vector only transfected HEK293T lysate) and FBXL16 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a FBXL16 Polyclonal Antibody (Product # PA5-58818).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FBXL16 in human cerebral cortex and rectum tissues using FBXL16 Polyclonal Antibody (Product # PA5-58818). Corresponding FBXL16 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FBXL16 in human rectum using FBXL16 Polyclonal Antibody (Product # PA5-58818) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FBXL16 in human cerebral cortex using FBXL16 Polyclonal Antibody (Product # PA5-58818) shows high expression.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 FBXL16 stabilized cyclin D1 protein by indirect interactions. (a, b) Ishikawa/MPA cells with shRNA-NC or shFBXL16 were pretreated with CHX for 90 min, and protein expression of cyclin D1 and cyclin E was analyzed by western blot at indicated times (left) and quantitatively analyzed (right). (c) Coimmunoprecipitation of FBXL16 and cyclin D1 in Ishikawa/MPA cells with the shRNA-NC or shFBXL16 group. Results are presented as mean +- SD. * P < 0.05. ** P < 0.01. *** P < 0.001.
