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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF1196 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Cystatin C Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Cystatin C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Cystatin C is observed and less than 5% cross-reactivity with recombinant human (rh) Fetuin A, rhFetuin B, rhCystatin A, rhCystatin B, rhCystatin S, and rhCystatin E/M is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P01034- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Nanoparticle Detection of Urinary Markers for Point-of-Care Diagnosis of Kidney Injury.
Cystatin E/M suppresses legumain activity and invasion of human melanoma.
Proteomic analysis of factors released from p21-overexpressing tumour cells.
Chung HJ, Pellegrini KL, Chung J, Wanigasuriya K, Jayawardene I, Lee K, Lee H, Vaidya VS, Weissleder R
PloS one 2015;10(7):e0133417
PloS one 2015;10(7):e0133417
Cystatin E/M suppresses legumain activity and invasion of human melanoma.
Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad Ø, Maelandsmo GM, Solberg R
BMC cancer 2010 Jan 15;10:17
BMC cancer 2010 Jan 15;10:17
Proteomic analysis of factors released from p21-overexpressing tumour cells.
Currid CA, O'Connor DP, Chang BD, Gebus C, Harris N, Dawson KA, Dunn MJ, Pennington SR, Roninson IB, Gallagher WM
Proteomics 2006 Jul;6(13):3739-53
Proteomics 2006 Jul;6(13):3739-53
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cystatin C by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, and human serum. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cystatin C by Western Blot. Western blot shows lysates of human placenta tissue and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1196) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cystatin C at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cystatin C in Human Breast. Cystatin C was detected in immersion fixed paraffin-embedded sections of human breast using 5 µg/mL Goat Anti-Human Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1196) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in cells of terminal ductules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
