PAB16580

antibody from Abnova Corporation

Targeting: SPRED1 FLJ33903, PPP1R147

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB16580

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB16580, RRID:AB_10677825

Product name

SPRED1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of SPRED1.

Storage

Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Spreds, inhibitors of the Ras/ERK signal transduction, are dysregulated in human hepatocellular carcinoma and linked to the malignant phenotype of tumors.

Yoshida T, Hisamoto T, Akiba J, Koga H, Nakamura K, Tokunaga Y, Hanada S, Kumemura H, Maeyama M, Harada M, Ogata H, Yano H, Kojiro M, Ueno T, Yoshimura A, Sata M
Oncogene 2006 Oct 5;25(45):6056-66

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Inhibition of angiogenesis by a mouse sprouty protein.

Lee SH, Schloss DJ, Jarvis L, Krasnow MA, Swain JL
The Journal of biological chemistry 2001 Feb 9;276(6):4128-33

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Spred is a Sprouty-related suppressor of Ras signalling.

Wakioka T, Sasaki A, Kato R, Shouda T, Matsumoto A, Miyoshi K, Tsuneoka M, Komiya S, Baron R, Yoshimura A
Nature 2001 Aug 9;412(6847):647-51

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of SPRED1 in mouse brain tissue lysate with SPRED1 polyclonal antibody (Cat # PAB16580) at (A) 1 and (B) 2 ug/mL .

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry of SPRED1 in human brain tissue with SPRED1 polyclonal antibody (Cat # PAB16580) at 2.5 ug/mL .

Validation comment

Immunohistochemistry