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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-22966 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLUT9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Cross-reactivity with human samples is very low.
- Concentration
- 1 mg/mL
Submitted references Hereditary hemochromatosis disrupts uric acid homeostasis and causes hyperuricemia via altered expression/activity of xanthine oxidase and ABCG2.
Ristic B, Sivaprakasam S, Narayanan M, Ganapathy V
The Biochemical journal 2020 Apr 30;477(8):1499-1513
The Biochemical journal 2020 Apr 30;477(8):1499-1513
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLUT9 using a polyclonal antibody (Product # PA5-22966).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GLUT9 in mouse kidney membrane. Sample was incubated in GLUT9 polyclonal antibody (Product # PA5-22966).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GLUT9 in mouse kidney section. Samples were incubated in GLUT9 polyclonal antibody (Product # PA5-22966) using a dilution of 1:200. The antibody generated an expected staining with more intensity towards the basolateral membranes of renal tubular epithelial cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Expression of Glut9 and MRP4 in wild type and Hfe -/- mouse liver and intestinal tract. ( A ) Quantitative PCR analysis of Glut9 mRNA in liver and intestinal segments of wild-type mouse. Data show mean values +- SEM relative to liver, which is taken as 1. * P < 0.05; *** P < 0.001. ( B ) Western blot for Glut9 protein levels in liver and intestinal segments of the wild-type mouse. Western blot band intensities were estimated using ImageJ software and the band intensities were normalized to the respective beta-tubulin band intensities. Please see Supplementary Figures S5 and S10 for full blot images. ( C ) Quantitative PCR of Glut9 mRNA in liver, jejunum, ileum, and colon of Hfe -/- mice and wild-type mice. Data show mean values of three mice per group +- SEM relative to control mice. ( D ) Western blot for Glut9 protein levels in Hfe -/- mouse and wild-type mouse liver, jejunum, ileum, and colon. Western blot band intensities were estimated using ImageJ software and the band intensities were normalized to the respective beta-actin band intensities. Please see Supplementary Figures S8, S9, and S11 for full blot images. ( E ) Quantitative PCR analysis of Mrp4 mRNA in liver and intestinal segments of wild-type mouse. Data show mean values +- SEM relative to liver, which is taken as 1. * P < 0.05; *** P < 0.001. ( F ) Quantitative PCR analysis of Mrp4 mRNA in liver, jejunum, ileum, and colon of Hfe -/- mice and wild-type mice. Data show mean values of three mice per gro
