NBP2-38443

antibody from Novus Biologicals

Targeting: PEX5 PTS1R, PXR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-38443

Provider

Novus Biologicals

Product name

Rabbit Polyclonal PEX5 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human PEX5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Spatiotemporal contact between peroxisomes and lipid droplets regulates fasting-induced lipolysis via PEX5.

Kong J, Ji Y, Jeon YG, Han JS, Han KH, Lee JH, Lee G, Jang H, Choe SS, Baes M, Kim JB
Nature communications 2020 Jan 29;11(1):578

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PEX5 Antibody [NBP2-38443] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PEX5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PEX5 Antibody [NBP2-38443] - Analysis using Anti-PEX5 antibody NBP2-38443 (A) shows similar pattern to independent antibody NBP1-87185 (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human testis shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining in human testis and pancreas tissues using anti-PEX5 antibody. Corresponding PEX5 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-PEX5 antibody NBP2-38443 (A) shows similar protein distribution across tissues to independent antibody NBP1-87185 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human colon.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human lymph node.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human cerebral cortex.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PEX5 Antibody [NBP2-38443] - Staining of human testis using Anti-PEX5 antibody NBP2-38443.