Explore
Validate
LearnA300-537A
antibody from Invitrogen Antibodies
Targeting: PDS5B
APRIN, AS3, CG008, FLJ23236, KIAA0979
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A300-537A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Pds5B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 0.20 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse Pds5B by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB in A; 1 mg for IP in B, 1/4 of IP reaction loaded), 293T (T, 50 µg for WB) and NIH3T3 (M, 50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-Pds5B antibody BL2352 (Cat. No. A300-537A) used in WB at 0.04 µg/ml (A) or 0.1 µg/ml (B) and for IP at 3 µg/mg lysate (B). Pds5B was also immunoprecipitated using rabbit anti-Pds5B antibody BL2353 (Cat. No. A300-538A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Pds5B by immunohistochemistry. Sample: FFPE sections of human skin carcinoma (left) and prostate carcinoma (right). Antibody: Affinity purified rabbit anti- Pds5B (Cat. No. A300-537A Lot1) used at a dilution of 1:200 (1µg/ml) and 1:80 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.
