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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NB100-755 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-755, RRID:AB_2299430
- Product name
- Rabbit Polyclonal APRIN Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The SUMO Isopeptidase SENP6 Functions as a Rheostat of Chromatin Residency in Genome Maintenance and Chromosome Dynamics.
Perturbing cohesin dynamics drives MRE11 nuclease-dependent replication fork slowing.
APRIN is a cell cycle specific BRCA2-interacting protein required for genome integrity and a predictor of outcome after chemotherapy in breast cancer.
Wagner K, Kunz K, Piller T, Tascher G, Hölper S, Stehmeier P, Keiten-Schmitz J, Schick M, Keller U, Müller S
Cell reports 2019 Oct 8;29(2):480-494.e5
Cell reports 2019 Oct 8;29(2):480-494.e5
Perturbing cohesin dynamics drives MRE11 nuclease-dependent replication fork slowing.
Carvajal-Maldonado D, Byrum AK, Jackson J, Wessel S, Lemaçon D, Guitton-Sert L, Quinet A, Tirman S, Graziano S, Masson JY, Cortez D, Gonzalo S, Mosammaparast N, Vindigni A
Nucleic acids research 2019 Feb 20;47(3):1294-1310
Nucleic acids research 2019 Feb 20;47(3):1294-1310
APRIN is a cell cycle specific BRCA2-interacting protein required for genome integrity and a predictor of outcome after chemotherapy in breast cancer.
Brough R, Bajrami I, Vatcheva R, Natrajan R, Reis-Filho JS, Lord CJ, Ashworth A
The EMBO journal 2012 Mar 7;31(5):1160-76
The EMBO journal 2012 Mar 7;31(5):1160-76
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: APRIN Antibody [NB100-755] - Detection of Human and Mouse Pds5B on HeLa whole cell lysate using NB100-755. Pds5B was also immunoprecipitated using rabbit anti-Pds5B antibody NB100-754.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: APRIN Antibody [NB100-755] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPds5b antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: APRIN Antibody [NB100-755] - Detection of human Pds5b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pds5b antibody NB100-755 used for IP at 3 ug per reaction. Pds5b was also immunoprecipitated by another rabbit anti-Pds5b antibody. For blotting immunoprecipitated Pds5b, NB100-755 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: APRIN Antibody [NB100-755] - Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti- Pds5B used at a dilution of 1:200 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: APRIN Antibody [NB100-755] - Section of human lung small cell carcinoma. Antibody: Affinity purified rabbit anti- Pds5B used at a dilution of 1:200 (1ug/ml). Detection: DAB
