MA5-15007

antibody from Invitrogen Antibodies

Targeting: PPP2R2A B55A, B55alpha, PR52A, PR55A, PR55alpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15007

Provider

Invitrogen Antibodies

Product name

PPP2R2A Monoclonal Antibody (F.722.1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with the B-prime (PR61), B-prime-prime or B-prime-prime-prime families of PP2A B subunits.

Reactivity

Human, Mouse, Rat, Drosophila

Host

Rabbit

Isotype

IgG

Antibody clone number

F.722.1

Vial size

100 µL

Concentration

104 µg/mL

Storage

-20°C

References

Submitted references

Direct Interaction of PP2A Phosphatase with GABA(B) Receptors Alters Functional Signaling.

Li X, Terunuma M, Deeb TG, Wiseman S, Pangalos MN, Nairn AC, Moss SJ, Slesinger PA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Apr 1;40(14):2808-2816

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of PPP2R2A was achieved by transfecting PC-3 with PPP2R2A specific validated siRNAs (Silencer® select Product # s608, s609). Western blot analysis (Fig. a) was performed using whole cell extracts from the PPP2R2A knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PPP2R2A Monoclonal Antibody (F.722.1) (Product # MA5-15007, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PPP2R2A.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of PC-3 (Lane 1), HEL 92.1.7 (Lane 2), K-562 (Lane 3), A549 (Lane 4), LNCap (Lane 5), HeLa (Lane 6), A-431 (Lane 7) and NIH/3T3 (Lane 8). The blot was probed with Anti-PPP2R2A Monoclonal Antibody (Product # MA5-15007, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 52 kDa band corresponding to PPP2R2A was observed across the cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PPP2R2A was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PPP2R2A Monoclonal Antibody (Product # MA5-15007) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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