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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX628528 - Provider product page
- Provider
- GeneTex
- Product name
- NIX/BNIP3L antibody [EPR4033], C-term
- Antibody type
- Monoclonal
- Host
- Rabbit
- Vial size
- 100 µl
Submitted references Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks.
Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy.
Domain Mapping of Heat Shock Protein 70 Reveals That Glutamic Acid 446 and Arginine 447 Are Critical for Regulating Superoxide Dismutase 2 Function.
Nuclear phosphorylated Dicer processes double-stranded RNA in response to DNA damage.
Negative pressure induces p120-catenin-dependent adherens junction disassembly in keratinocytes during wound healing.
Schistosoma mansoni α1,3-fucosyltransferase-F generates the Lewis X antigen.
Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis.
SNX27 and SORLA Interact to Reduce Amyloidogenic Subcellular Distribution and Processing of Amyloid Precursor Protein.
Inactivation of Tsc2 in Mesoderm-Derived Cells Causes Polycystic Kidney Lesions and Impairs Lung Alveolarization.
Release of Infectious Hepatitis C Virus from Huh7 Cells Occurs via a trans-Golgi Network-to-Endosome Pathway Independent of Very-Low-Density Lipoprotein Secretion.
FOXK2 transcription factor suppresses ERα-positive breast cancer cell growth through down-regulating the stability of ERα via mechanism involving BRCA1/BARD1.
Application of an Integrated Omics Approach for Identifying Host Proteins That Interact With Odontoglossum ringspot virus Capsid Protein.
Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration.
TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain.
An anti-inflammatory NOD-like receptor is required for microglia development.
Burger K, Schlackow M, Gullerova M
Nucleic acids research 2019 Apr 23;47(7):3467-3484
Nucleic acids research 2019 Apr 23;47(7):3467-3484
Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy.
Shen T, Cai LD, Liu YH, Li S, Gan WJ, Li XM, Wang JR, Guo PD, Zhou Q, Lu XX, Sun LN, Li JM
Journal of hematology & oncology 2018 Jul 17;11(1):95
Journal of hematology & oncology 2018 Jul 17;11(1):95
Domain Mapping of Heat Shock Protein 70 Reveals That Glutamic Acid 446 and Arginine 447 Are Critical for Regulating Superoxide Dismutase 2 Function.
Afolayan AJ, Alexander M, Holme RL, Michalkiewicz T, Rana U, Teng RJ, Zemanovic S, Sahoo D, Pritchard KA Jr, Konduri GG
The Journal of biological chemistry 2017 Feb 10;292(6):2369-2378
The Journal of biological chemistry 2017 Feb 10;292(6):2369-2378
Nuclear phosphorylated Dicer processes double-stranded RNA in response to DNA damage.
Burger K, Schlackow M, Potts M, Hester S, Mohammed S, Gullerova M
The Journal of cell biology 2017 Aug 7;216(8):2373-2389
The Journal of cell biology 2017 Aug 7;216(8):2373-2389
Negative pressure induces p120-catenin-dependent adherens junction disassembly in keratinocytes during wound healing.
Huang CH, Hsu CC, Chen CP, Chow SE, Wang JS, Shyu YC, Lu MJ
Biochimica et biophysica acta 2016 Sep;1863(9):2212-20
Biochimica et biophysica acta 2016 Sep;1863(9):2212-20
Schistosoma mansoni α1,3-fucosyltransferase-F generates the Lewis X antigen.
Mickum ML, Rojsajjakul T, Yu Y, Cummings RD
Glycobiology 2016 Mar;26(3):270-85
Glycobiology 2016 Mar;26(3):270-85
Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis.
Wang G, Liu X, Gaertig MA, Li S, Li XJ
Proceedings of the National Academy of Sciences of the United States of America 2016 Mar 22;113(12):3359-64
Proceedings of the National Academy of Sciences of the United States of America 2016 Mar 22;113(12):3359-64
SNX27 and SORLA Interact to Reduce Amyloidogenic Subcellular Distribution and Processing of Amyloid Precursor Protein.
Huang TY, Zhao Y, Li X, Wang X, Tseng IC, Thompson R, Tu S, Willnow TE, Zhang YW, Xu H
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Jul 27;36(30):7996-8011
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Jul 27;36(30):7996-8011
Inactivation of Tsc2 in Mesoderm-Derived Cells Causes Polycystic Kidney Lesions and Impairs Lung Alveolarization.
Ren S, Luo Y, Chen H, Warburton D, Lam HC, Wang LL, Chen P, Henske EP, Shi W
The American journal of pathology 2016 Dec;186(12):3261-3272
The American journal of pathology 2016 Dec;186(12):3261-3272
Release of Infectious Hepatitis C Virus from Huh7 Cells Occurs via a trans-Golgi Network-to-Endosome Pathway Independent of Very-Low-Density Lipoprotein Secretion.
Mankouri J, Walter C, Stewart H, Bentham M, Park WS, Heo WD, Fukuda M, Griffin S, Harris M
Journal of virology 2016 Aug 15;90(16):7159-70
Journal of virology 2016 Aug 15;90(16):7159-70
FOXK2 transcription factor suppresses ERα-positive breast cancer cell growth through down-regulating the stability of ERα via mechanism involving BRCA1/BARD1.
Liu Y, Ao X, Jia Z, Bai XY, Xu Z, Hu G, Jiang X, Chen M, Wu H
Scientific reports 2015 Mar 5;5:8796
Scientific reports 2015 Mar 5;5:8796
Application of an Integrated Omics Approach for Identifying Host Proteins That Interact With Odontoglossum ringspot virus Capsid Protein.
Lin PC, Hu WC, Lee SC, Chen YL, Lee CY, Chen YR, Liu LY, Chen PY, Lin SS, Chang YC
Molecular plant-microbe interactions : MPMI 2015 Jun;28(6):711-26
Molecular plant-microbe interactions : MPMI 2015 Jun;28(6):711-26
Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration.
Wu JC, Chen YC, Kuo CT, Wenshin Yu H, Chen YQ, Chiou A, Kuo JC
Scientific reports 2015 Dec 18;5:18476
Scientific reports 2015 Dec 18;5:18476
TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain.
Yan S, Wang CE, Wei W, Gaertig MA, Lai L, Li S, Li XJ
Human molecular genetics 2014 May 15;23(10):2678-93
Human molecular genetics 2014 May 15;23(10):2678-93
An anti-inflammatory NOD-like receptor is required for microglia development.
Shiau CE, Monk KR, Joo W, Talbot WS
Cell reports 2013 Dec 12;5(5):1342-52
Cell reports 2013 Dec 12;5(5):1342-52
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GFP antibody detects GFP protein by Western blot analysis.A. 5 µg 293T whole cell extractB. 5 µg 293T whole cell extract expressing GFP-tagged protein 12 % SDS-PAGEGFP antibody (GTX628528) dilution: 1:5000
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELISA detection of GFP using GTX628528 for capture and GTX113617 for detection.Coating Ab: mouse anti-GFP (GTX628528, 2μg/well )Sample: GFP proteinDetected Ab: rabbit anti-GFP (GTX113617)Conjμgated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GFP-tag antibody detects GFP-recombinant protein by immunofluorescent analysis. Sample: GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody (GTX628528) diluted at 1:2000. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 ?g cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 £gg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 £gg of GFP tag antibody (GTX628528) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628528) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
