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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- 313402 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#313402, RRID:AB_314995
- Product name
- Purified anti-human/mouse ZAP-70
- Antibody type
- Monoclonal
- Antigen
- GST-fusion protein corresponding to residues 282-307 of human ZAP-70
- Description
- ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
Submitted references CD38 gene polymorphisms contribute to genetic susceptibility to B-cell chronic lymphocytic leukemia: evidence from two case-control studies in Polish Caucasians.
Jamroziak K, Szemraj Z, Grzybowska-Izydorczyk O, Szemraj J, Bieniasz M, Cebula B, Giannopoulos K, Balcerczak E, Jesionek-Kupnicka D, Kowal M, Kostyra A, Calbecka M, Wawrzyniak E, Mirowski M, Kordek R, Robak T
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2009 Mar;18(3):945-53
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2009 Mar;18(3):945-53
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-ZAP-70 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated
