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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AGC-021-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EAAT1 (GLAST) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021) (1:500).3,4. Anti-EAAT1 (GLAST) (extracellular) Antibody , preincubated with EAAT1/GLAST (extracellular) Blocking Peptide (#BLP-GC021).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021) (1:500).3,4. Anti-EAAT1 (GLAST) (extracellular) Antibody , preincubated with EAAT1/GLAST (extracellular) Blocking Peptide (#BLP-GC021).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of EAAT1 in human U-87 MG cells - Cell surface detection of EAAT1 in live intact human U-87 MG glioblastoma cells. A. Extracellular staining of cells using Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021), (1:100) followed by goat Anti-rabbit-Alexa-Fluor-594 secondary Antibody (red). B. Live image of the cells. C. Merge of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of EAAT1 in rat hippocampus - Immunohistochemical staining was performed in perfusion-fixed free floating rat brain sections (frozen) using Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021), (1:100). A. EAAT1 staining (green) is particularly intense along the subgranular layer (arrows). B. Staining with mouse anti glial fibrillary acidic protein (red). C. Merged picture confirms presence of densely packed astrocytes along the subgranular layer. DAPI is used as a general cell marker (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection ofEAAT1by indirect flow cytometry in live intacthumanMEG-01 megakaryocyticleukemiacells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells+ Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021), (2.5μg) + goat- Anti-rabbit-FITC.
