Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
Reference
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- Product number
- H00084444-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00084444-M01, RRID:AB_538351
- Product name
- Mouse Monoclonal DOT1L Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DOT1L - DOT1-like, histone H3 methyltransferase (S. cerevisiae)
- Reactivity
- Human, Feline
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Incidence of methylated histones H3K4 and H3K79 in cat germinal vesicles is regulated by specific nuclear factors at the acquisition of developmental competence during the folliculogenesis.
Phillips TC, Wildt DE, Comizzoli P
Journal of assisted reproduction and genetics 2016 Jun;33(6):783-94
Journal of assisted reproduction and genetics 2016 Jun;33(6):783-94
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DOT1L Antibody (6A6) [H00084444-M01] - DOT1L monoclonal antibody (M01), clone 6A6. Analysis of DOT1L expression in LNCaP.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: DOT1L Antibody (6A6) [H00084444-M01] - Detection limit for recombinant GST tagged DOT1L is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: DOT1L Antibody (6A6) [H00084444-M01] - Analysis of monoclonal antibody to DOT1L on HeLa cell . Antibody concentration 10 ug/ml.