A300-953A

antibody from Invitrogen Antibodies

Targeting: DOT1L DOT1, KIAA1814, KMT4

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-953A

Provider

Invitrogen Antibodies

Product name

DOT1L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DOT1L by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody A300-953A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). DOT1L was also immunoprecipitated by rabbit anti-DOT1L antibody A300-954A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human DOT1L by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L (Cat. No. A300-953A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Localization of DOT1L Binding Sites by ChIP-sequencing. Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody A300-953A and analyzed by DNA sequencing. The figure illustrates the peak distribution of DOT1L binding within a 250 Kb region of chromosome 10 as detected using anti-DOT1L antibody A300-953A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.