MAB6546

antibody from R&D Systems

Targeting: DOT1L DOT1, KIAA1814, KMT4

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6546

Provider

R&D Systems

Product name

Human DOT1L Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human DOT1L in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PCDH-8, rhNME-3, or rhWISP-3 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q8TEK3


Isotype

IgG

Antibody clone number

653613

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

DOT1L safeguards cartilage homeostasis and protects against osteoarthritis.

Monteagudo S, Cornelis FMF, Aznar-Lopez C, Yibmantasiri P, Guns LA, Carmeliet P, Cailotto F, Lories RJ
Nature communications 2017 Jun 19;8:15889

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

DOT1L in Human Lung. DOT1L was detected in immersion fixed paraffin-embedded sections of human lung using Human DOT1L Monoclonal Antibody (Catalog # MAB6546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.