HPA003552

antibody from Atlas Antibodies

Targeting: STXBP6 amisyn, HSPC156

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA003552

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003552, RRID:AB_10603343

Product name

Anti-STXBP6

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NKKPTQASITKVKQFEGSTSFVRRSQWMLEQLRQV
NGIDPNGDSAEFDLLFENAFDQWVASTASEKCTFF
QILHHTCQRYLTDRKPEFINCQSKIMGGNSILHSA
ADSVTSAVQKASQALNERGERLGRAEEKTEDLK


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines Caco-2 and PC-3 using Anti-STXBP6 antibody. Corresponding STXBP6 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and STXBP6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415452).

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human caudate shows strong positivity in neuronal cells.

Sample type

HUMAN