Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB123 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD23/Fc epsilon RII Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD23/Fc epsilon RII in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Trapping IgE in a closed conformation by mimicking CD23 binding prevents and disrupts FcεRI interaction.
Jabs F, Plum M, Laursen NS, Jensen RK, Mølgaard B, Miehe M, Mandolesi M, Rauber MM, Pfützner W, Jakob T, Möbs C, Andersen GR, Spillner E
Nature communications 2018 Jan 2;9(1):7
Nature communications 2018 Jan 2;9(1):7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of CD23/Fc epsilon RII in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD23/Fc epsilon RII Monoclonal Antibody (Catalog # MAB123) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.