PA5-120638

antibody from Invitrogen Antibodies

Targeting: PLD3 HU-K4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120638

Provider

Invitrogen Antibodies

Product name

PLD3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PLD3 in various cell lines using a PLD3 Polyclonal antibody (Product # PA5-120638) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of PLD3 in L929 cells using a PLD3 Polyclonal antibody (Product # PA5-120638). Blue: DAPI for nuclear staining.