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ImmunocytochemistryAntibody data
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- Antigen structure
- References [2]
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- Western blot [2]
- ELISA [1]
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- Product number
- H00003753-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003753-M01, RRID:AB_829277
- Product name
- Mouse Monoclonal KCNE1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. KCNE1 (5B12)
- Reactivity
- Human, Rat, Canine, Guinea Pig, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Chronic in vivo angiotensin II administration differentially modulates the slow delayed rectifier channels in atrial and ventricular myocytes.
[Ca2+]i elevation and oxidative stress induce KCNQ1 protein translocation from the cytosol to the cell surface and increase slow delayed rectifier (IKs) in cardiac myocytes.
Zankov DP, Salloum FN, Jiang M, Tseng GN
Heart rhythm 2019 Jan;16(1):108-116
Heart rhythm 2019 Jan;16(1):108-116
[Ca2+]i elevation and oxidative stress induce KCNQ1 protein translocation from the cytosol to the cell surface and increase slow delayed rectifier (IKs) in cardiac myocytes.
Wang Y, Zankov DP, Jiang M, Zhang M, Henderson SC, Tseng GN
The Journal of biological chemistry 2013 Dec 6;288(49):35358-71
The Journal of biological chemistry 2013 Dec 6;288(49):35358-71
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KCNE1 Antibody (5B12) [H00003753-M01] - analysis of KCNE1 expression in transfected 293T cell line by KCNE1 monoclonal antibody (M01), clone 5B12. Lane 1: KCNE1 transfected lysate (14.7 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KCNE1 Antibody (5B12) [H00003753-M01] - Analysis of KCNE1 over-expressed 293 cell line, cotransfected with KCNE1 Validated Chimera RNAi ( Cat # H00003753-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KCNE1 monoclonal antibody (M01), clone 5B12 (Cat # H00003753-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: KCNE1 Antibody (5B12) [H00003753-M01] - Detection limit for recombinant GST tagged KCNE1 is approximately 3ng/ml as a capture antibody.
