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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- TA324606 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal RCAN1 Antibody (N-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal RCAN1 Antibody (N-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RCAN1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- RCAN1 Antibody (N-term) (Cat. #TA324606) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RCAN1 antibody detected the RCAN1 protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of RCAN1 (arrow) using rabbit polyclonal RCAN1 Antibody (N-term) (Cat. #TA324606). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RCAN1 gene.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of A431 cell stained with RCAN1 Antibody (N-term)(Cat#TA324606).A431 cells were incubated with RCAN1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . RCAN1 immunoreactivity is localized to Cytoplasm significantly.
- Validation comment
- IF
