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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-77313 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CaV3.2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.9 mg/mL
- Storage
- -20°C
Submitted references Mechano-signaling via Piezo1 prevents activation and p53-mediated senescence of muscle stem cells.
Peng Y, Du J, Günther S, Guo X, Wang S, Schneider A, Zhu L, Braun T
Redox biology 2022 Jun;52:102309
Redox biology 2022 Jun;52:102309
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat DRG lysates with CaV3.2 polyclonal antibody (Product # PA5-77313) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CaV3.2 in paraformaldehyde-fixed and permeabilized rat dorsal root ganglion. A, D) Samples were probed with CaV3.2 polyclonal antibody (Product # PA5-77313) at a dilution of 1:200, and incubated with goat-anti-rabbit-AlexaFluor-555 and Hoechst. B, E) Shows nuclei stained image. C) Shows merged images of Panels A and B. F) Shows merged images of Panels D and E. Magnification: A-C: x20, D-F: x100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CaV3.2 in frozen rat dorsal root ganglion. Samples were probed with CaV3.2 polyclonal antibody (Product # PA5-77313) using a dilution of 1:50, and incubated with Hoechst.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CaV3.2 in frozen rat dorsal root ganglion. Samples were probed with CaV3.2 polyclonal antibody (Product # PA5-77313) using a dilution of 1:50, and incubated with Hoechst.
