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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- HPA026816 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA026816, RRID:AB_10602089
- Product name
- Anti-SEC11C
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SEC11C, Gene description: SEC11 homolog C (S. cerevisiae), Alternative Gene Names: SEC11L3, SPC21, SPCS4C, Validated applications: IHC, WB, Uniprot ID: Q9BY50, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Submitted references A CRISPR screen defines a signal peptide processing pathway required by flaviviruses
Zhang R, Miner J, Gorman M, Rausch K, Ramage H, White J, Zuiani A, Zhang P, Fernandez E, Zhang Q, Dowd K, Pierson T, Cherry S, Diamond M
Nature 2016;535(7610):164-168
Nature 2016;535(7610):164-168
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and SEC11C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403241).
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subsets of lymphoid cells outside reaction centra.
- Sample type
- HUMAN
