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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-55427 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEC11C Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MVRAGAVGAH LPASGLDIFG DLKKMNKRQL YYQV
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEC11C in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a SEC11C Polyclonal Antibody (Product # PA5-55427).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEC11C in control (vector only transfected HEK293T lysate) and SEC11C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SEC11C Polyclonal Antibody (Product # PA5-55427).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SEC11C in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a SEC11C Polyclonal Antibody (Product # PA5-55427).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SEC11C in human lymph node shows strong cytoplasmic positivity in subsets of lymphoid cells outside reaction centra. Samples were probed using a SEC11C Polyclonal Antibody (Product # PA5-55427).
