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- Antibody Data
- Antigen structure
- References [3]
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- Product number
- AF4736 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Bone marrow stromal cell antigen 1/CD157 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CD157 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse CD157 is observed.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references NAD(+) augmentation restores mitophagy and limits accelerated aging in Werner syndrome.
Human canonical CD157/Bst1 is an alternatively spliced isoform masking a previously unidentified primate-specific exon included in a novel transcript.
Binding of CD157 protein to fibronectin regulates cell adhesion and spreading.
Fang EF, Hou Y, Lautrup S, Jensen MB, Yang B, SenGupta T, Caponio D, Khezri R, Demarest TG, Aman Y, Figueroa D, Morevati M, Lee HJ, Kato H, Kassahun H, Lee JH, Filippelli D, Okur MN, Mangerich A, Croteau DL, Maezawa Y, Lyssiotis CA, Tao J, Yokote K, Rusten TE, Mattson MP, Jasper H, Nilsen H, Bohr VA
Nature communications 2019 Nov 21;10(1):5284
Nature communications 2019 Nov 21;10(1):5284
Human canonical CD157/Bst1 is an alternatively spliced isoform masking a previously unidentified primate-specific exon included in a novel transcript.
Ferrero E, Lo Buono N, Morone S, Parrotta R, Mancini C, Brusco A, Giacomino A, Augeri S, Rosal-Vela A, García-Rodríguez S, Zubiaur M, Sancho J, Fiorio Pla A, Funaro A
Scientific reports 2017 Nov 21;7(1):15923
Scientific reports 2017 Nov 21;7(1):15923
Binding of CD157 protein to fibronectin regulates cell adhesion and spreading.
Morone S, Augeri S, Cuccioloni M, Mozzicafreddo M, Angeletti M, Lo Buono N, Giacomino A, Ortolan E, Funaro A
The Journal of biological chemistry 2014 May 30;289(22):15588-601
The Journal of biological chemistry 2014 May 30;289(22):15588-601
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human CD157 by Western Blot. Western blot shows lysates of human kidney (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD157 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4736) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD157 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
