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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-26800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DCLK1 Monoclonal Antibody (OTI1F8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1F8
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A Combinatorial MAP Code Dictates Polarized Microtubule Transport.
Monroy BY, Tan TC, Oclaman JM, Han JS, Simó S, Niwa S, Nowakowski DW, McKenney RJ, Ori-McKenney KM
Developmental cell 2020 Apr 6;53(1):60-72.e4
Developmental cell 2020 Apr 6;53(1):60-72.e4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-DCLK1 Monoclonal Antibody (OTI1F8) (Product # MA5-26800) and a 80kDa band corresponding to DCLK1 was observed in Mouse Pup Brain, Mouse Brain and Rat Brain, but not in Mouse Pup Kidney and Mouse Kidney. Additional band at ~25kDa was observed in Mouse tissues corresponding to free floating IgG. Whole cell lysate (30ug lysate) of Mouse Pup Brain (Lane 1), Mouse Pup Kidney (Lane 2), Mouse Brain (Lane 3), Mouse Kidney (Lane 4), and Rat Brain (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:2000 dilution) detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human embryonic cerebellum tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a DCLK1 monoclonal antibody (Product # MA5-26800) at a dilution of 1:500.
