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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-87021 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-87021, RRID:AB_1201179
- Product name
- Rabbit Polyclonal ESCO2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Promiscuous Targeting of Cellular Proteins by Vpr Drives Systems-Level Proteomic Remodeling in HIV-1 Infection.
Localization and regulation of murine Esco2 during male and female meiosis.
Identification and expression of potential regulators of the mammalian mitotic-to-meiotic transition.
Greenwood EJD, Williamson JC, Sienkiewicz A, Naamati A, Matheson NJ, Lehner PJ
Cell reports 2019 Apr 30;27(5):1579-1596.e7
Cell reports 2019 Apr 30;27(5):1579-1596.e7
Localization and regulation of murine Esco2 during male and female meiosis.
Evans EB, Hogarth C, Evanoff RM, Mitchell D, Small C, Griswold MD
Biology of reproduction 2012 Sep;87(3):61
Biology of reproduction 2012 Sep;87(3):61
Identification and expression of potential regulators of the mammalian mitotic-to-meiotic transition.
Hogarth CA, Mitchell D, Evanoff R, Small C, Griswold M
Biology of reproduction 2011 Jan;84(1):34-42
Biology of reproduction 2011 Jan;84(1):34-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ESCO2 Antibody [NB100-87021] - HeLa cells lysis buffer. Antibody: Affinity purified rabbit anti-ESCO2 antibody) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ESCO2 Antibody [NB100-87021] - Detection of Human ESCO2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ESCO2 antibody NB100-87021 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ESCO2 Antibody [NB100-87021] - Detection of human ESCO2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ESCO2 antibody NB100-87021 (lot 2) used for IP at 6 ug per reaction. ESCO2 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-ESCO2 antibody from Company B. For blotting immunoprecipitated ESCO2, NB100-87021 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
