CABT-B9207

antibody from Creative Diagnostics

Targeting: FKBP1A FKBP-12, FKBP1, FKBP12, FKBP12C, PKC12, PPIASE

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

CABT-B9207

Provider

Creative Diagnostics

Product name

Mouse anti-Human FKBP12 monoclonal antibody, clone 9/GLCQ23

Antibody type

Monoclonal

Description

Transforming growth factor-ÉÇ binds to the TGFÉÇ family of heteromeric serine/threonine transmembrane receptors (type I and type II). Following binding of TGFÉÇ, the type II receptor (TÉÇR-II) phosphorylates the type I receptor (TÉÇR-I) which, in turn, conveys the signal. Since TÉÇR-I and TÉÇR-II can interact without the stimulation of TGFÉÇ, leading to unwanted activation, a regulatory mechanism exists. In a yeast genetic screen, immunophilin FKBP12 was associated with the type I receptor. Studies including co-immunoprecipiation, deletion, and point mutations confirmed this interaction. FKBP12 inhibits TÉÇR-II mediated phosphorylation of TÉÇR-I, inhibiting activation. FKBP12 binds via its rapamycin/Leu-Pro binding pocket to the Leu-Pro sequence adjacent to the phosphorylation site of TÉÇR-I. This interaction is blocked by the addition of macrolides, rapamycin, and FK506. Furthermore, mutations in the binding sites of FKBP12 and TÉÇR-I abrogates the binding and results in activation of the receptor without the addition of TGFÉÇ. Thus, FKBP12 is a regulatory protein for TÉÇR-I and TÉÇR-II-mediated signaling.

Reactivity

Human, Mouse, Canine, Chicken/Avian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

9/GLCQ23

Vial size

50 _g

Concentration

250 Ã×g/ml

Storage

Store undiluted at -20°C.