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Western blot
Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00253260-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00253260-M01, RRID:AB_547715
- Product name
- Mouse Monoclonal Rictor Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RICTOR - rapamycin-insensitive companion of mTOR
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references In situ characterization of the mTORC1 during adipogenesis of human adult stem cells on chip.
Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains.
GNAQ and BRAF mutations show differential activation of the mTOR pathway in human transformed cells.
mTOR pathway overactivation in BRAF mutated papillary thyroid carcinoma.
Nuclear forkhead box O1 controls and integrates key signaling pathways in hepatocytes.
Wu X, Schneider N, Platen A, Mitra I, Blazek M, Zengerle R, Schüle R, Meier M
Proceedings of the National Academy of Sciences of the United States of America 2016 Jul 19;113(29):E4143-50
Proceedings of the National Academy of Sciences of the United States of America 2016 Jul 19;113(29):E4143-50
Interaction between NBS1 and the mTOR/Rictor/SIN1 complex through specific domains.
Wang JQ, Chen JH, Chen YC, Chen MY, Hsieh CY, Teng SC, Wu KJ
PloS one 2013;8(6):e65586
PloS one 2013;8(6):e65586
GNAQ and BRAF mutations show differential activation of the mTOR pathway in human transformed cells.
Pópulo H, Tavares S, Faustino A, Nunes JB, Lopes JM, Soares P
PeerJ 2013;1:e104
PeerJ 2013;1:e104
mTOR pathway overactivation in BRAF mutated papillary thyroid carcinoma.
Faustino A, Couto JP, Pópulo H, Rocha AS, Pardal F, Cameselle-Teijeiro JM, Lopes JM, Sobrinho-Simões M, Soares P
The Journal of clinical endocrinology and metabolism 2012 Jul;97(7):E1139-49
The Journal of clinical endocrinology and metabolism 2012 Jul;97(7):E1139-49
Nuclear forkhead box O1 controls and integrates key signaling pathways in hepatocytes.
Naïmi M, Gautier N, Chaussade C, Valverde AM, Accili D, Van Obberghen E
Endocrinology 2007 May;148(5):2424-34
Endocrinology 2007 May;148(5):2424-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.Lane 1: RICTOR transfected lysate(29.8 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rictor Antibody (1F3) [H00253260-M01] - RICTOR monoclonal antibody (M01), clone 1F3 Analysis of RICTOR expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Rictor Antibody (1F3) [H00253260-M01] - Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rictor Antibody (1F3) [H00253260-M01] - Analysis of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
