NBP1-51645

antibody from Novus Biologicals

Targeting: RICTOR AVO3, KIAA1999, MGC39830, PIA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-51645

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-51645, RRID:AB_11022534

Product name

Mouse Monoclonal Rictor Antibody

Antibody type

Monoclonal

Description

Ammonium sulfate precipitation.

Reactivity

Human, Mouse, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Loss of mTOR signaling affects cone function, cone structure and expression of cone specific proteins without affecting cone survival.

Ma S, Venkatesh A, Langellotto F, Le YZ, Hall MN, Rüegg MA, Punzo C
Experimental eye research 2015 Jun;135:1-13

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Inhibition of EGFR-AKT axis results in the suppression of ovarian tumors in vitro and in preclinical mouse model.

Loganathan S, Kandala PK, Gupta P, Srivastava SK
PloS one 2012;7(8):e43577

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Rictor Antibody (7B3) [NBP1-51645] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Rictor Antibody (7B3) [NBP1-51645] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on Jurkat cells with Rictor Antibody (7B3) NBP1-51645AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.