PAB13054

antibody from Abnova Corporation

Targeting: MED28 DKFZP434N185, EG1, magicin

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB13054

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB13054, RRID:AB_10552049

Product name

MED28 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of MED28.

Storage

Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Identification of host proteins required for HIV infection through a functional genomic screen.

Brass AL, Dykxhoorn DM, Benita Y, Yan N, Engelman A, Xavier RJ, Lieberman J, Elledge SJ
Science (New York, N.Y.) 2008 Feb 15;319(5865):921-6

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Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation.

Beyer KS, Beauchamp RL, Lee MF, Gusella JF, Näär AM, Ramesh V
The Journal of biological chemistry 2007 Nov 2;282(44):32152-7

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A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.

Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC
Molecular cell 2004 Jun 4;14(5):685-91

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of MED28 in human brain tissue lysate with MED28 polyclonal antibody (Cat # PAB13054) at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human brain tissue with 2.5 ug/mL MED28 polyclonal antibody (Cat # PAB13054).

Validation comment

Immunohistochemistry