PAB22921

antibody from Abnova Corporation

Targeting: MED28 DKFZP434N185, EG1, magicin

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22921

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22921, RRID:AB_10961928

Product name

MED28 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant MED28.

Antigen sequence

VIKEDVSELRNELQRKDALVQKHLTKLRHWQQVLE
DINVQHKKPADIPQGSLAYLEQASANIPAPLKPT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with MED28 polyclonal antibody (Cat # PAB22921).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with MED28 polyclonal antibody (Cat # PAB22921) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human hippocampus with MED28 polyclonal antibody (Cat # PAB22921) shows moderate cytoplasmic and nuclear membranous positivity in neurons at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)