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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX115249 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115249, RRID:AB_10623070
- Product name
- VPS4B antibody [N3C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references CK2 involvement in ESCRT-III complex phosphorylation.
Salvi M, Raiborg C, Hanson PI, Campsteijn C, Stenmark H, Pinna LA
Archives of biochemistry and biophysics 2014 Mar 1;545:83-91
Archives of biochemistry and biophysics 2014 Mar 1;545:83-91
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE GTX115249 diluted at 1:3000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VPS4B antibody [N3C3] detects VPS4B protein at nucleus and endosome by immunofluorescent analysis.Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VPS4B protein stained by VPS4B antibody [N3C3] (GTX115249) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VPS4B antibody [N3C3] detects VPS4B protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: VPS4B protein stained by VPS4B antibody [N3C3] (GTX115249) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- VPS4B antibody [N3C3] detects VPS4B protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS4B antibody [N3C3] (GTX115249) dilution: 1:500.
