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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-30316 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VPS4B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T.
- Concentration
- 0.48 mg/mL
Submitted references TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons.
Schwenk BM, Hartmann H, Serdaroglu A, Schludi MH, Hornburg D, Meissner F, Orozco D, Colombo A, Tahirovic S, Michaelsen M, Schreiber F, Haupt S, Peitz M, Brüstle O, Küpper C, Klopstock T, Otto M, Ludolph AC, Arzberger T, Kuhn PH, Edbauer D
The EMBO journal 2016 Nov 2;35(21):2350-2370
The EMBO journal 2016 Nov 2;35(21):2350-2370
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using VPS4B Polyclonal Antibody (Product # PA5-30316). Sample (30 µg of whole cell lysate). Lane A: 293T. 10% SDS PAGE. VPS4B Polyclonal Antibody (Product # PA5-30316) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of VPS4B showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a VPS4B polyclonal antibody (Product # PA5-30316) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of VPS4B was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B Polyclonal Antibody (Product # PA5-30316) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of VPS4B was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B Polyclonal Antibody (Product # PA5-30316) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- VPS4B Polyclonal Antibody detects VPS4B protein at nucleus and endosome by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS4B protein stained by VPS4B Polyclonal Antibody (Product # PA5-30316) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- VPS4B Polyclonal Antibody detects VPS4B protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS4B Polyclonal Antibody (Product # PA5-30316) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
