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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- TA805169 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA805169, RRID:AB_2627612
- Product name
- MSLN mouse monoclonal antibody, clone OTI1G8 (formerly 1G8)
- Antibody type
- Monoclonal
- Description
- MSLN mouse monoclonal antibody, clone OTI1G8 (formerly 1G8)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MSLN
- Isotype
- IgG
- Antibody clone number
- OTI1G8
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSLN (RC202532, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSLN.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MSLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805169)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- MSLN Luminex ELISA with 1G8 Capture (TA805169) and 3E10 Detection (TA805261) Antibodies. Substrate used: Recombinant Human MSLN domain expressed in E.coli.
- Validation comment
- LMNX
