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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA5-11918 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mesothelin Monoclonal Antibody (5B2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-11918 targets Mesothelin in IHC (P) applications and shows reactivity with Human samples.
- Antibody clone number
- 5B2
- Concentration
- Conc. Not Determined
Submitted references Diagnostic value of IMP3 and mesothelin in differentiating pancreatic ductal adenocarcinoma from chronic pancreatitis.
Generation of Potent T-cell Immunotherapy for Cancer Using DAP12-Based, Multichain, Chimeric Immunoreceptors.
Mesothelin enhances invasion of ovarian cancer by inducing MMP-7 through MAPK/ERK and JNK pathways.
Primary localized malignant biphasic mesothelioma of the liver in a patient with asbestosis.
Primary localized malignant biphasic mesothelioma of the liver in a patient with asbestosis.
The angiogenesis inhibitor thrombospondin-1 inhibits acute cutaneous hypersensitivity reactions.
Biomarkers in Diagnosis of pancreatic carcinoma in fine-needle aspirates.
Biomarkers in Diagnosis of pancreatic carcinoma in fine-needle aspirates.
Ibrahim DA, Abouhashem NS
Pathology, research and practice 2016 Apr;212(4):288-93
Pathology, research and practice 2016 Apr;212(4):288-93
Generation of Potent T-cell Immunotherapy for Cancer Using DAP12-Based, Multichain, Chimeric Immunoreceptors.
Wang E, Wang LC, Tsai CY, Bhoj V, Gershenson Z, Moon E, Newick K, Sun J, Lo A, Baradet T, Feldman MD, Barrett D, Puré E, Albelda S, Milone MC
Cancer immunology research 2015 Jul;3(7):815-26
Cancer immunology research 2015 Jul;3(7):815-26
Mesothelin enhances invasion of ovarian cancer by inducing MMP-7 through MAPK/ERK and JNK pathways.
Chang MC, Chen CA, Chen PJ, Chiang YC, Chen YL, Mao TL, Lin HW, Lin Chiang WH, Cheng WF
The Biochemical journal 2012 Mar 1;442(2):293-302
The Biochemical journal 2012 Mar 1;442(2):293-302
Primary localized malignant biphasic mesothelioma of the liver in a patient with asbestosis.
Sasaki M, Araki I, Yasui T, Kinoshita M, Itatsu K, Nojima T, Nakanuma Y
World journal of gastroenterology 2009 Feb 7;15(5):615-21
World journal of gastroenterology 2009 Feb 7;15(5):615-21
Primary localized malignant biphasic mesothelioma of the liver in a patient with asbestosis.
Sasaki M, Araki I, Yasui T, Kinoshita M, Itatsu K, Nojima T, Nakanuma Y
World journal of gastroenterology 2009 Feb 7;15(5):615-21
World journal of gastroenterology 2009 Feb 7;15(5):615-21
The angiogenesis inhibitor thrombospondin-1 inhibits acute cutaneous hypersensitivity reactions.
Velasco P, Huegel R, Brasch J, Schröder JM, Weichenthal M, Stockfleth E, Schwarz T, Lawler J, Detmar M, Lange-Asschenfeldt B
The Journal of investigative dermatology 2009 Aug;129(8):2022-30
The Journal of investigative dermatology 2009 Aug;129(8):2022-30
Biomarkers in Diagnosis of pancreatic carcinoma in fine-needle aspirates.
Jhala N, Jhala D, Vickers SM, Eltoum I, Batra SK, Manne U, Eloubeidi M, Jones JJ, Grizzle WE
American journal of clinical pathology 2006 Oct;126(4):572-9
American journal of clinical pathology 2006 Oct;126(4):572-9
Biomarkers in Diagnosis of pancreatic carcinoma in fine-needle aspirates.
Jhala N, Jhala D, Vickers SM, Eltoum I, Batra SK, Manne U, Eloubeidi M, Jones JJ, Grizzle WE
American journal of clinical pathology 2006 Oct;126(4):572-9
American journal of clinical pathology 2006 Oct;126(4):572-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Mesothelin was achieved by transfecting NIH:OVCAR-3 with Mesothelin specific siRNAs (Silencer® select Product # S19967, S19968). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the Mesothelin knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Mesothelin Monoclonal Antibody (5B2) (Product # MA5-11918, 1:1000 dilution ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20000 dilution). Densitometric analysis of this Western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Mesothelin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-Mesothelin Monoclonal Antibody (5B2) (Product # MA5-11918) and a ~50 kDa band corresponding to Mesothelin was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1) and NIH:OVCAR-3 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human mesothelioma stained with Mesothelin antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells.
