Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB110-85538 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-85538, RRID:AB_1199375
- Product name
- Mouse Monoclonal Mesothelin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Mesothelin expression in human lung cancer.
New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA.
Ho M, Bera TK, Willingham MC, Onda M, Hassan R, FitzGerald D, Pastan I
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Mar 1;13(5):1571-5
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Mar 1;13(5):1571-5
New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA.
Onda M, Willingham M, Nagata S, Bera TK, Beers R, Ho M, Hassan R, Kreitman RJ, Pastan I
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Aug 15;11(16):5840-6
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Aug 15;11(16):5840-6
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538] - Detection of mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng of CDC25-Fc. Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane. Primary antibody was used at 1 g/ml followed by reaction with ALP goat anti-mouse IgG and BCI:5840-6.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Mesothelin Antibody (MB-G10) [NB110-85538] - Human tissue sections treated by antigen retrieval methods. Primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin.Cancer Res. 11(16):5840-6.
