24563-1-AP

antibody from Invitrogen Antibodies

Targeting: NCAPG CAP-G, FLJ12450, hCAP-G, YCG1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

24563-1-AP

Provider

Invitrogen Antibodies

Product name

NCAPG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TLHCEGTEI NSDDEQESKE VEETATAKNV LKLLSDFLDS EVSELRTGAA EGLAKLMFSG LLVSSRILSR LILLWYNPVT EEDVQLRHCL GVFFPVFAYA SRTNQECFEE AFLPTLQTLA NAPASSPLAE IDITNVAELL VDLTRPSGLN PQAKTSQDYQ ALTVHDNLAM KICNEILTSP CSPEIRVYTK ALSSLELSSH LAKDLLVLLN EILEQVKDRT CLRALEKIKI QLEKGNKEFG DQAEAAQDAT LTTTTFQNED EKNKEVYMTP LRGVKATQAS KSTQLKTNRG QRKVTVSART NRRCQTAEAD SESDHEVPEP ESEMKMRLPR RAKTAALEKS KLNLAQFLNE DLS (664-1015 aa encoded by B C068467)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.35 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 24563-1-AP ( NCAPG Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 24563-1-AP (NCAPG Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 24563-1-AP (NCAPG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 24563-1-AP (NCAPG Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 24563-1-AP ( NCAPG Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 24563-1-AP ( NCAPG Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NCAPG (IP:24563-1-AP, 4ug; Detection:24563-1-AP 1:1000) with HEK-293 cells lysate 3200ug.